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Protein and Peptide Separation

Protein and peptide separation is used regularly to decrease the complexity in each fraction on the mass spec. This greatly increases the dynamic range of sensitivity in the instrument, and is required for very complex samples, such as total cell lysates, and quantitative experiments.

The Core routinely uses basic reverse phase (bRP) or strong cation exchange columns (SCX) with on of our off-line HPLC's to fraction complex samples prior to running them. This is required for complex mixtures, especially for quantitative experiments. Other types of columns (MARS, C4, HILIC, Size Exclusion) are available for specific projects if required.

We are also equip to use the Bio-Rad Mini Prep Cell to separate purified proteins by their molecular weight and collect them off-gel in a fraction collector.

Location

The Johns Hopkins Mass Spectrometry and Proteomic Facility

725 N. Wolfe Street
Wood Basic Science Building, Room 504
Baltimore, MD 21205

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