user_mobilelogo

Services

The mission of the Mass Spectrometry and Proteomics Core is to help investigators identify proteins and their modifications that are differentially expressed cells, tissues or body fluids, track interactions with binding partners during changes in signal transduction, identify proteolytic cleavage sites and map post-translational modifications through the following services.

  • Consultation: Pre and Post-analysis
  • Sample preparation:  Buffer exchange; Column Chromatography; Proteolytic digestion; Stable isotope labeling
  • Protein Identification:  Proteins in solution, in gel bands or spots, or in complex protein mixtures
  • Protein Modifications:  Acetylatiion, AMPylation, citrullination, glycosylation, phosphorylation, proline hydroxylation, nitrosation, ubiquitination and novel cleavage sites, etc
  • Protein Quantification:  Tandem Mass Tags (TMT), Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ), Absolute Quantification (AQUA), Protein Standard Absolute Quantification (PSAQ).
  • High Resolution Mass Analysis of Intact Protein:  Protein characterization and their modifications
  • Biostatical/Bioinformatic Anlaysis:  PCA analysis, Volcano plots, Ingenuity bases gene ontology pathway analysis; Bioinformatics consultation.

 

Location

The Johns Hopkins Mass Spectrometry and Proteomic Facility

725 N. Wolfe Street
Wood Basic Science Building, Room 504
Baltimore, MD 21205

Newsletter Signup

Sign up for all of our updates.
Please wait