The mission of the Johns Hopkins University (JHU) School of Medicine (SOM) Mass Spectrometry and Proteomics Facility is to accelerate discovery by giving investigators access to cutting edge technologies in mass spectrometry and proteomics. To keep Johns Hopkins scientists competitive, new technologies will be incorporated into the facility as soon as possible to meet your needs. The facility is open to all Hopkins investigators and off-site investigators on a fee-for-service basis. The core staff is able to assist you in determining the best approach to analyzing your sample. Be sure to read the instructions for submitting samples and download the sample submission form. Whether your sample is complex or isolated, in solution or in a gel band, we get you identified!
Protein Identification: from in solution or in gel, complex protein mixtures or purified samples
Protein Quantification: Isobaric tagging for relative and absolute quantitation (iTRAQ), Tandem mass tags (TMT), Difference gel electrophoresis (DIGE), 180-Labeling, Absolute Quantification (AQUA), Protein Standard Absolute Quantification (PSAQ).
Protein Characterization: acetylation, glycosylation, phosphorylation, nitrosation, ubiquitination and novel cleavage sites. We can also characterize intact proteins and their modifications using high resolution intact mass.
Training: MALDI/TOF, Gel electrophoresis and Typhoon imager training is available.